Welcome to HTS Corrector
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HTS Corrector software is designed to detect systematic errors in HTS assays and correct experimental datasets
Departement d'Informatique
Authors: Dmytro Kevorkov, Vladimir Makarenkov, Contact:
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References:
Abstract:
High-throughput screening (HTS) is a novel and efficient
technology for drug discovery. It allows for screening of more than 100,000
compounds a day per screen and requires effective procedures for quality
control. We have developed a method for evaluating a background surface of an
HTS assay; it can be used to correct raw HTS data. This correction is necessary
to take into account systematic errors that may affect the procedure of hit
selection. The described method allows one to analyze experimental HTS data and
determine trends and local fluctuations of the corresponding background
surfaces. For a large amount of plates of the same assay, the deviations of the
background surface from a plane are caused by systematic errors. Their influence
can be minimized by the subtraction of the systematic background from the raw
data. Two experimental HTS assays from the ChemBank database were examined in
this paper. The systematic error present in these data was estimated and removed
from them. It enabled us to correct the hit selection procedure for both assays.
Kevorkov D. and Makarenkov V.
Statistical
analysis of systematic errors in high-throughput screening,
Journal of Biomolecular Screening, 10: 557-567, 2005.
Kevorkov D. and Makarenkov V. Quality control and data correction in high-throughput screening, Proceedings of SFC 2005, pp. 159-163, Montreal, Canada.
Makarenkov V., Kevorkov D., Zentilli P., Gagarin A., Malo N. and Nadon R. HTS-Corrector: software for the statistical analysis and correction of experimental high-throughput screening data, Bioinformatics, 2006, in press.
